| A rapid, low-cost and simple technique has been developed for the determination of aflatoxin B1(AFB1) in eggs and livers
using high-performance liquid chromatography (HPLC) with UV detection. In this study, the presence of AFB1 was
investigated in 150 eggs and 50 chicken livers from the local market of Tabriz, Iran. AFB1
was extracted with a mixture of acetonitrile: water (80:20) and cleaned up by dispersive liquid-liquid microextraction which is a very economical, fast and sensitive method. AFB1was quantified by HPLC-UV without the need for any complex derivatization in samples to enhance the detection. The results showed that 72% of the liver and 58% of the egg samples were contaminated with AFB1ranging from 0.30 to 16.36 µg kg̶1. limit of detection and limit of quantification for AFB1were 0.08 and 0.28 µg kg1, respectively. The proposed method is suitable e for fast analysing of AFB1in egg and liver samples. |