چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background and aim.Endocan is a novel endothelium-derived proteoglycan and mayplay a role in endothelial cells activity under diabetic conditions. Here, we evaluatedthe effect of high glucose concentration (30 mmol glucose) on endocan level in presenceor absence of metformin in human umbilical vein endothelial cells (HUVECs).Methods.Cells were incubated with 30 mmol glucose for 72 h. High glucose content,metformin (2.5 to 500 mmol) and compound C (10 mmol) effects were assessed on cellviability. HUVECs migration was studied by scratch test. The changes in endocan expres-sion and protein level were evaluated by RT-PCR, ELISA and flow cytometry assays. Gri-ess reaction was used to measure NO levels. Functional activity of endothelial cells wasmonitored related to lipoprotein lipase activity using Dil-Ac-LDL uptake. p-AMPK/AMPK ratio was assessed by western blotting.Results.Cells viability significantly was reduced under high glucose condition(p!0.05). 30 mmol glucose inhibited HUVECs migration, whereas these features wereimproved by 50 mmol metformin (p!0.05). Endocan transcription and protein levelswere increased in diabetic HUVECs exposed to metformin (p!0.05). Metforminincreased NO production in HUVECs under high glucose condition (p!0.001). Met-formin increased LDL uptake capacity under high glucose condition (p!0.05). Theaddition of compound C blunted these effects. Western blot analysis confirmed the in-crease of p-AMPK/AMPK ratio in metformin-treated cells.Conclusion.Data demonstrated that metformin could promote angiogenic potential ofendothelial cells which its reduction is a main cause in the development of diabetic footulcer, probably by the regulation of endocan dynamics under high glucose condi-tion.Ó2019 IMSS. Published by Elsevier Inc |
| نویسنده | نفر چندم مقاله |
|---|---|
| المیرا زلالی | اول |
| آیسا رضابخش | دوم |
| رضا رهبرقاضی | پنجم |
| علیرضا گرجانی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| ddd.pdf | 1398/07/29 | 2675903 | دانلود |
