| Objective: To determine the synergists action of piperonyl butoxide (PBO) and S,S,Stributyl
phosphorotrithioate (DEF) on toxicity of carbamate insecticides against Blattella
germanica in Tehran city.
Methods: In the current study, German cockroach strains were collected from several
hospitals and dormitories in Tehran. At the beginning, different concentrations of
bendiocarb and carbaryl (insecticides belong to carbamate group) were determined by
surface contact on a susceptible strain. Then, the level of susceptibility and type of
resistance mechanisms in the collected strains from contaminated sites to the aforementioned
insecticides were studied by using PBO and DEF synergists with different
insecticide ratios to synergist (1:0, 1:1, 1:2, 1:3).
Results: The DEF synergist along with bendiocarb and carbaryl completely eliminated
the resistance in all strains but PBO did not completely eliminate the resistance in the
strains of Mofid, Alvand, Valiasr hospitals and Shariati dormitory. Generally, the impact
of DEF was observed in the removing resistance more than PBO.
Conclusions: In most of these strains, resistance to bendiocarb and carbaryl is
completely eliminated by DEF, showing a very high role of estraze enzymes in resistance
to bendiocarb and carbaryl. But in most strains PBO does not remove the resistance
because other mechanisms, such as reduced cuticle penetration and insensitivity to the
acetylcholine esterase enzyme, may be involved |