| The reversed-phase preparative high performance liquid
chromatographic purification of the methanol extract of
the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded
five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl
cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin
3-O-glucoside (3), cyanidin 3-O-(2G-xylosyl)-rutinoside (4)
and cyanidin 3-O-rutinoside (5). They showed considerable
free-radical-scavenging properties in the 2,2-diphenyl-
1-picrylhydrazyl (DPPH) assay with the RC50 values
of 9.29 ´ 10–6, 9.33 ´ 10–6, 8.31 ´ 10–6, 8.96 ´ 10–6 and 9.55
´ 10–6 mol L–1, respectively. The structures of these compounds
were elucidated by various chemical hydrolyses
and spectroscopic means. The total anthocyanin content
was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside
basis. |