چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Colorectal cancer (CRC) remains a universal and lethal cancer owing to metastatic and relapsing disease. Currently, the role of microRNAs has been checked in tumorigeneses. Numerous studies have revealed that between the tumor suppressor miRNAs, the reduced expression of miR-146a-5p and -193a5p in several cancers including CRC tissues are related with tumor progression and poor prognosis of patients. The purpose of this study is to examine the role of miR-146 a-5p and -193 a-5p in CRC cell cycle progression. The miR-193a-5p and -146 a-5p mimics were transfected into HT-29 CRC cells via JetPEI transfection reagent and their impact was assessed on p53, cyclin B, and NF-kB gene expression. The inhibitory effect of these miRNAs on cell cycle was assessed by flow cytometry. The consequence of miR-193a-5p and miR-146 a5p on the protein expression level of MDM2 was assessed by western blotting. Restoration of miR‐193a‐5p and miR-146 a-5p expression induces G1 arrest in colorectal cancer through targeting of The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Results: miR193a-5p and -146a-5p regulated the expression of MDM2 protein and p53, cyclin B, and NF-kB gene expression in CRC cells. Treatment of HT29 cells with miRNA-146a-5p and -193a-5p induced G1 cell cycle arrest. Conclusion: The findings of our study suggest that miR146a-5p and -193a-5p may act as a potential tumor suppressor by their influence on cell cycle progression in CRC cells. Thus, miRNA-146a-5p and -193a-5p restoration may be recommended as a potential therapeutic goal in the treatment of CRC patients . |
| نویسنده | نفر چندم مقاله |
|---|---|
| سعید نورعلیایی | اول |
| الهام باغبانی | دوم |
| لیلی عاقبتی | سوم |
| امیر باغبانزاده | چهارم |
| داریوش شانه بندی | پنجم |
| وحید خاضع شاهگلی | پنجم |
| امیر علی مختارزاده | ششم |
| بهزاد برادران | هفتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 335-Restoration of miR-193a-5p and miR-146 a-5p Expression Induces G1.pdf | 1399/03/03 | 849745 | دانلود |
| rezayat nameh.jpeg | 1399/07/14 | 1369920 | دانلود |
