چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Abstract Purpose Free-living amoeba (FLA) including Acanthamoebaspp., Balamuthia mandrillaris, and Naegleriaare among the soil-born parasites. There are reports of FLA-related keratitis with a history of contact with soil and dust sources, particularly among the farmers. Due to lack of the previous studies on the farmland soils and a limited number of researches conducted on recreational soils in Iran, the present study was conducted. Methods A total of 93 soil samples including farming lands and recreational places were tested for the presence of Acanthamoebaspp. Balamuthia mandrillaris, Naegleria, and Vermamoebausing morphological key and sequencing-based tools. Pathogenicity of Acanthamoebapositive strains was also evaluated. To verify genetic associations and taxonomic status of isolated amoeba, a phylogenetic tree was built by MEGA 5.05 software inferred by the 18S rRNA gene based on maximum likelihood algorithm. Results Overall, 28 samples (30%) were contaminated with potentially pathogenic FLA, and according to the sequencing data, 17 strains were successfully sequenced. The isolated Acanthamoebabelonged to T2, T4, T5, mixed T4 and T5 contaminations, and T11. ITS sequencing revealed the occurrence of one strain of Naegleria canariensis. Four strains of Vermamoeba vermiformiswere also confrmed. Morphological survey and PCR assay failed to show any positive results for Balamuthia mandrillaris. Pathogenic potential of the Acanthamoebastrains showed that T2, T4, and T11 genotypes were highly pathogenic, whereas T5 genotypes demonstrated lower pathogenic potential. Conclusion The results indicate that soil could be a serious hazard to human health, and therefore, further studies are expected to investigate the source of infection in patients developing FLA-related diseases. The present study is the frst to investigate FLA in the farmland soils in Iran and the frst to report the presence of N. canariensisin the country. |
| نویسنده | نفر چندم مقاله |
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| عادل اسپوتین | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Pazoki2019_Article_IsolationAndPhylogeneticAnalys.pdf | 1398/07/10 | 1799973 | دانلود |
