| Recently, enormous attention has been focused on the development of protein-molecularly imprinted polymers
(MIPs). In this sense, bovine serum albumin (BSA) is well regarded as a favorite template in various MIPs-based
biochemical/analytical assays mainly due to its low price, easy availability, and high structural homology to human
serum albumin (HSA). Equally, the implications of BSA in the pathology of different human-related disorders necessitate the development of methods for its precise detection in biological samples. Accordingly, the current review
seeks to provide an update on the design, synthesis, and characterization of the developed MIPs which have used
BSA as template protein. Also, the recognition and quantifcation of BSA in different real samples using the prepared
MIPs are discussed. Additionally, main strategies, such as surface imprinting, epitope-MIPs, microcontact imprinting
and other methods to overcome the problems associated with the molecular printing of BSA are discussed here. The
fnal discussion provides a comparative exploration of different approaches developed, emphasizing their relative
advantages and disadvantages and underlining developments and possible future directions. |