چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
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| عزت اله فتحی | اول |
| راحله فرحزادی | دوم |
| عنوان | متن |
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| خلاصه مقاله | MESENCHYMAL STEM CELLS INDUCED EARLY APOPTOSIS IN B65-NEUROGENIC DIFFERENTIATED CELLS AS IN VITRO CELL LINE MODEL FOR ALZHEIMERS DISEASE Ezzatollah Fathi1, *, Raheleh Farahzadi2, Ilja Vietor 3 1 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. 2 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 3 Division of Cell Biology, Biocenter, Medical University Innsbruck, Innrain 80-82, A-6020, Innsbruck, Austria. Corresponding Author: Ezzatollah Fathi, PhD (e-mail: ez.fathi@tabrizu.ac.ir; Postal Code: 5166616471; Tel: +98-41-333923, Fax: +98-41-333923. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, Iran). Background and Aim: Mesenchymal stem cells (MSCs) as undifferentiated multipotent cells are of particular interest due their potential clinical use in regenerative medicine. Stem cell-based therapies cast a new hope for neurodegenerative disease such as Alzheimer’s disease (AD) treatment as a replacement or regeneration strategy. Neurodegenerative diseases are groups of acute or/and chronic diseases that depending on operating condition cause to loss of neuroglia as well as neural cells. There is not absolute treatment due to the complete lack of understanding of the factors and conditions involved in them. Understanding the cellular mechanisms involved in Alzheimer's patients experience memory impairment is essential to design and determine the effectiveness of new drugs and therapies. The aim of this study is to explore the influence of secreted cytokines of MSCs on apoptosis of B65-neurogenic differentiated cell line as in vitro cell line model for AD. Materials & Methods: For this purpose, MSCs were isolated from bone marrow and co-cultured with B65-neurogenic differentiated cell (1:10) for 5 days. At the end of co-culture period, B65 differentiated cells were collected and subjected to Annexin/PI assessment. Results: It was shown that MSCs cause to significant induction of early apoptosis in B65-neurogenic differentiated cell. In detail, it was found that 37.3% of the cells were in early apoptotic stage (Annexin+, PI-), which was 2.91 times higher than that of the control group (12.8%). Conclusion: In this study, we reported that MSCS could be used for apoptosing B65-neurogenic differentiated cell as model in cell-based therapy for Alzheimer's Disease. |
| کلمات کلیدی | Key Words: Mesenchymal stem cell, B65-neurogenic differentiated cells, Alzheimers disease |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract3.jpg | 1398/06/16 | 617435 | دانلود |
| Certificate.jpg | 1398/06/16 | 127655 | دانلود |
