چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| عزت اله فتحی | اول |
| راحله فرحزادی | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Key Words: L-carnitine, C-kit+ progenitor cells, Cardiogenesis |
| خلاصه مقاله | L-CARNITINE CONTRIBUTE TO PROMOTE CARDIOGENESIS OF C-KIT+ PROGENITOR CELLS-DERIVED BONE MARROW Ezzatollah Fathi1, *, Raheleh Farahzadi2, Ilja Vietor3, Sara Javanmardi1 1 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. 2 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 3 Division of Cell Biology, Biocenter, Medical University Innsbruck, Innrain 80-82, A-6020, Innsbruck, Austria. Corresponding Author: Ezzatollah Fathi, PhD (e-mail: ez.fathi@tabrizu.ac.ir; Postal Code: 5166616471; Tel: +98-41-333923, Fax: +98-41-333923. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, Iran). Background and Aim: C-kit+ Bone marrow progenitor cells have recently gained much attention due to the therapeutic effects following their administration into the infarcted heart as evidenced by animal studies and by a recent clinical trial. However, injecting these cells in the heart is associated with poor differentiation into specialized cardiac cell types and with rapid death of the engrafted cells. With the ultimate goal of advancing cardiac stem cell therapy, we sought to facilitate the differentiation of c-kit+ Bone marrow progenitor cells into cardiac cell types (e.g. cardiomyocytes, smooth muscle cells, endothelial cells and cardiac fibroblasts) by overexpressing selected cardiac transcription factors in vitro. On the other hand, the effective role of L-carnitine as strong and effective antioxidant in improved cardiac function has been proven. Materials & Methods: For this purpose, mononuclear cells were isolated from rat bone marrow and c-kit+ (CD117) cells were separated with MACs. In the following, c-kit+ cells were treated with L-carnitine for 7, 14 and 21 days and gene expression of cardiac TFs, including Gata4 and TBX5 were evaluated by Real time PCR. Results: It was shown that L-carnitine cause to significant increase in gene expression of Gata4 and TBX5. In detail, it was found that L-carnitine promoted the cardiogenesis of c-kit+ progenitor cells. Conclusion: It was concluded that L-carnitine could be used for facilitating the differentiation of c-kit+ Bone marrow progenitor cells into cardiac cell types with the aim of cell-based therapy. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
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| Abstarct2.jpg | 1398/06/16 | 375854 | دانلود |
| Certificate.jpg | 1398/06/16 | 127655 | دانلود |
