Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk
Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk
نویسندگان: بهزاد جعفری , سیاوش دستمالچی
کلمات کلیدی: Affinity purification
Alkaline phosphatase
Sodium phosphate salt
Sodium orthovanadate
Bovine milk
Magnetic nanocarriers.
نشریه: 55107 , 3 , 175 , 2019
| نویسنده ثبت کننده مقاله |
سیاوش دستمالچی |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
مرکز تحقیقات بیوتکنولوژی(زیست فناوری) |
| کد مقاله |
68235 |
| عنوان فارسی مقاله |
Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk |
| عنوان لاتین مقاله |
Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk |
| ناشر |
4 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
خیر |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
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| Modified Fe3O4 magnetic nanoparticles (magnetic nanocarrier) technology have found the proper place in separation and purification techniques, such as protein and enzyme purification, mostly due to its easy and fast
operational procedure by using an external permanent magnet. Herein, Fe3O4 magnetic nanoparticles were
prepared, and surface modification was performed with vanadate and phosphate salts to yield four various
model of magnetic nanocarriers. Affinity ligands which are used for immobilization on the nanocarriers leading
to the development of appropriative nanocarriers for the affinity separation of alkaline phosphatase from the
bovine milk. The findings showed that the use of sodium hexametaphosphate affinity ligand attached to the
carrier with an 18-atom linker leads to better separation of alkaline phosphatase from the bovine milk with 14.1-
fold purification efficiency. All results confirmed that our designed nanocarriers can purify alkaline phosphatase
using a fast and low-cost approach. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| Magnetic iron oxide nanoparticles modified.pdf | 1398/05/14 | 2078651 | دانلود |