| This study aimed to determine phenolic compounds of Artemisia spicigera (family
Asteraceae) growing in East-Azarbaijan province of Iran. 20%, 40 % and 60% SPE
fractions of methanolic extract of A. spicigera, were subjected to reversed phase preparative
HPLC, with the mobile phase consisted of methanol and water. Structural identiication of
phytochemicals by spectroscopic methods including UV and NMR spectroscopy, yielded
4, 6-di methoxy acetophenone-2-O-β-D-glucopyranoside from 20%, 5-methoxyluteolin
7-O-β-D-glucopyranoside, luteolin and chrysoeriol 7-O-β-D-glucopyranoside from 40% and
5-methoxy luteolin from 60% SPE fractions. Although within identiied pure compounds,
luteolin was the only phenolic reported from some other species of Artemisia, but occurrence of
remained identiied phenolics in this study, was irstly reported from Artemisia genus. Further
phytochemical investigations were proposed in order to isolate some other active fractions and
pure compounds |