| A novel moderately halophilic, Gramstain negative and aerobic bacterium, designated
strain TBZ21T
, was isolated from a water sample of
Urmia Lake, Iran. Cells were observed to be nonmotile rods with no flagellum, showing positive
catalase and oxidase reactions. Strain TBZ21T was
found to grow at 10–40 C (optimum, 30 C), at pH
7–10 (optimum, pH 8) and in the presence of 1–22%
(optimum, 10%). The major fatty acids were identified
as C19:0 cyclo x8c, C16:0, Summed features 3 (C13:0
3-OH and/or iso-C15:1 H) and 8 (C18:1 x7c and/or C18:1
x6c) and C12:0 3-OH. The major polar lipids were
identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phosphoaminolipid. The genomic DNA G?C
content of strain TBZ21T was determined to be
63.3 mol%. Phylogenetic analyses based on 16S
rRNA gene sequences indicated that strain TBZ21T
belongs to the genus Halomonas, and shows high
sequence similarities to Halomonas fontilapidosi
5CRT (99.43%), Halomonas ventosae AL12T
(98.64%), Halomonas sediminicola CPS11T
(98.63%) and Halomonas aestuarii Hb3T (98.08%),
and has low similarities (below 98.0%) with other
members of the genus. The values of DNA–DNA
relatedness between strain TBZ21T and the closely
related strains H. fontilapidosi LMG 24455T and H. |