بررسی اثر محافظتی نانوذرات جامد لیپیدی حاوی L-carnitine بر استرس اکسیداتیو تولید شده در سلول های HUVEC توسط پراکسیدهیدروژن

مجریان: مرتضی اسکندانی

خلاصه روش اجرا: L-carnitine-loaded SLNs (LC-SLNs) will be prepared for the first time in this investigation using Theobroma cacao (cocoa) butter to improve the protective role of LC against intracellular oxidative stress damages throughout increasing its bioavailability. Various physical properties (e.g., morphology, size, zeta potential) as well as encapsulation efficiency (EE%), drug loading (DL), drug content (DC), in vitro release, and kinetics of release of the prepared LC-SLNs will be investigated. Besides, the safety of the prepared SLNs will be evaluated against the human umbilical vein endothelial cells (HUVECs) and the protective role of LC against intracellular oxidative stress damages and apoptosis occurrence will be evaluated in H2O2-induced oxidative stress in HUVECs through the quantification of the level intracellular reactive oxygen species (ROS).