چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهران راغب | اول |
| بابک الیاسی فر | دوم |
| آزیتا دیلمقانی | سوم |
| سمیه حلاج نژادی | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Study of antimicrobial activity of Bacillus megaterium isolated from honey Mehran Ragheb1*, Babak Elyasifar1, Azita Dilmaghani1, Somayeh Hallaj nezhadi 2 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. 2Department of pharmaceutical and food control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Abstract Introduction Nowadays, multi antibiotic-resistant pathogens are an important health problem in medicine. Therefore finding novel sources of antimicrobial compounds is essential to introduce effective agents against resistant pathogen microorganisms. One of the microorganisms producing antimicrobial is Bacillus. The present study looks to antimicrobial activity of Bacillus megaterium, which isolated from honey from south of Iran. Method The B. megaterium was cultured into a broth medium. After incubation the bacterium was grown for 48h at 30 °C. The bacterial extracts were filtered through a 0.22 µm pore filter membrane after centrifugation of bacterial culture at 5000 rpm for 10 min. Six microorganism were studied as human pathogenic organisms and 8 were used as plant pathogenic. All bacterial strains were cultured on nutrient agar and fungal strains were cultured on MEA media.The microbes were dissolved in sterile distilled water and compared to 0.5 McFarland control. The plates were then incubated at 30 °C for 24 hours and the diameter of the zone of inhibition was measured. Results The antimicrobial test showed that B. megaterium has ability to produce antimicrobial against plant pathogens. This isolate showed the highest inhibition zone against Fusarium oxysporum. Discussion The Discovery of novel antibiotic is essential subject because probability of multiple resistances on pathogenic microorganisms is very high. This is the first report on the antibiotic production ability of B. megaterium. |
| کلمات کلیدی | Bacillus megaterium, antimicrobial activity, Honey |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 20181112135954124_0001.jpg | 1398/04/02 | 1527163 | دانلود |
| journal.docx | 1398/04/02 | 14549 | دانلود |
