چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Legionella pneumophila is a causative agent of Pontiac fever and Legionaries' disease and its identification. Also L. pnumophila is one of the threatening pathogens that has many challenges for its rapid and specific detection. The present study is aimed to designing an appropriate DNA based bioassay to identification of L. pneumophila. To achieve ideal biosensor, gold nanoparticles supported by chitosan and cetyltrimethyl ammonium bromide (CTAB) was electrodeposited on the surface of gold electrode. Cyclic voltammetry technique was used to coating gold nanostructure. Also Field emission scanning electron microscope (FE-SEM) and energy dispersive spectroscopy (EDX) were applied for morphological and elementary analysis investigation. The prepared substrate was used for immobilization of pDNA and hybridization by complementary target sequence (cDNA). Therefore, an innovative method to the sensitive detection of target DNA based was proposed. Used probe sequence was. Obtained results demonstrated that in the optimum conditions, linear range was 1 μM to 1 fM and low limit of quantification (LLOQ) was 1 f-molar. Engineered DNA biosensor not only shows good sensitivity and selectivity but also displays rapid performance in detection of L. pneumophila. Findings of this study indicate that for designing an ideal biosensor for this pathogen, we can use the wide range of nanomaterials to increase the sensitivity and accuracy of the future biosensors. |
| نویسنده | نفر چندم مقاله |
|---|---|
| احمد موبدکرکج | اول |
| محمد حسن زاده محله | دوم |
| پریناز بابائی | سوم |
| محمد آقازاده | چهارم |
| امیر علی مختارزاده | پنجم |
| محمد آهنگرزاده رضایی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 186.pdf | 1398/04/01 | 3588136 | دانلود |
