چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| ویدا ابراهیمی | اول |
| وحیده طرح ریز | دوم |
| محمد سعید حجازی | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction and background: Polycyclic Aromatic Hydrocabons (PAHs), are kind of genotoxic contaminations with high concentrations in many industrial sites and are considered as one of the environmental concerns since stability against degradation and potential of bioaccumulation in food chain. Bacteria are specially involved in their biodegradation among microorganisms which are being progressively isolated from marine habitats. This study was aimed to find biodegrading bacteria from Caspian Sea with ability to use some PAHs as only carbon source. Methods: Water samples were taken from estuary of Shahrood river which then flows into the Caspian Sea. 21 bacteria were isolated from water samples on sea water medium. Among these isolates, the ability to use some PAHs as the only carbon source, were tested by growth intensity on 3 ONR7a supplemented solid medias with the only carbon source of PAHs including Naphthalene, Phenanthrene and Anthracene respectively in comparison with control medias (without organic carbon sources). Isolates which could powerfully grow on all of them were subjected for 16S rDNA sequencing. Phylogenetic tree was constructed using neighbor-joining method in MEGA6 software. Biochemical tests containing API 20E and API 20NE kits and some lab-made tests were performed. Results: Six isolates were able to grow on all of supplemented ONR7a media containing: SF2, SFR1, SFR2, SFR3, SS1 and SS2. Biochemical tests were performed on these 6 bacteria. Based on phylogenetic results it is recommended that the isolates belong to Shewanella, Photobacterium, Micobacterium and Pseudomonas. Discussion and Conclusion: By taking into consideration all results and searching references, it can be concluded that bacteria of Caspian Lake have an efficient role in biodegradation of PAHs. By separating the PAH degrading bacteria from Caspian Lake, it has been clarified that part of the purification process of the lake is done by these bacteria. |
| کلمات کلیدی | polycyclic aromatic hydrocarbons (PAHs), 16S rRNA, Caspian Sea |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Study of biodiversity of some marine bacteria with polycyclic aromatic hydrocarbon (PAH) degradation potential from Caspian Sea .pdf | 1398/03/13 | 238416 | دانلود |
| Study of biodiversity of some marine bacteria with polycyclic aromatic hydrocarbon (PAH) degradation potential from Caspian Sea.pdf | 1398/03/13 | 716481 | دانلود |
