چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| وحیده طرح ریز | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | One of the major methods to reduce various pollutants is applying biologic method which is known as bioremediation. The aim of the present study was tried to analyze the existence of naphthalene-degradation bacteria phenotypically and genotypically in Qurugöl Lake, located in East Azerbaijan Province in Iran. At the end of each rainy season samples were taken. These samples were incubated in a specific medium where naphthalene was the only carbonic source of energy for providing the poly aromatic hydrocarbons (PAHs) energy. The single colonies were isolated and the primary phonotypic and biochemical tests were carried out. for genotypic analysis, their 16S rRNA genes were separated and sequenced. Considering the fact, that the decomposing bacteria of PAHs compounds, which were taken from the lake were isolated, it became obvious that part of polluting process is done by these bacteria. The significant bacteria in this case are bacillus sp. and closely related type strains include B pumilus (99.9%) Which are capable of decomposing PAHs efficiently in 5% salt concentrations also Shewanella sp. and closely related type strains include Sh. Xiamenensis (99.1%) which are capable of decomposing PAHs efficiently in 1/5% salt concentration. t is recommended that isolated RCRI7(=RCRI8) represents a novel strain of the species Sh. xiamenensis and The GenBank/EMBL accession number for the 16S. rRNA gene sequences of strain RCRI7 is GQ988720 The level of DNA–DNA relatedness betweenstrain RCRI7 and phylogenetically the closest related strain, Shewanella xiamenensis JCM.T was 51%. |
| کلمات کلیدی | Naphthalene, Qurugöl Lake, bacillus sp, shewanella sp, 16S rRNA gene |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 1.pdf | 1398/03/11 | 246637 | دانلود |
| 1..JPG | 1398/03/11 | 727412 | دانلود |
