| خلاصه مقاله | Plants of genus Clinopodium have been used in different cultures as traditional
medicines. Due to the importance of medicinal properties of genus Clinopodium, C.
umbrosum was selected for phytochemical analysis along with evaluation of its
antioxidant property. The aerial parts of C. umbrosum were extracted with petroleum
ether, chloroform, and methanol. Later, the methanol extract was fractionated via solid
phase extraction (SPE), vacuum liquid chromatography (VLC), and reversed phase high
performance liquid chromatography (HPLC). Consequently, structures of the isolated
compounds were analyzed through spectral analysis 1D NMR and 2D NMR data.
Additionally, the antioxidant property of C. umbrosum methanol extract together with its
phenolic and flavonoids content were assessed. Structure elucidation of the purified
compounds revealed presence of two triterpene saponins and a caffeic acid derivative in
C. umbrosum methanol extract. Moreover, the RC50 value for free radical scavenging
activity of the methanol extract was determined as 38.52 µg/ml and values for the total
phenolic and flavonoids contact were calculated as 5.14 g gallic acid equivalent and 4.25
g quercetin equivalent per 100 g of dried plant material, respectively. Overall, the present
study is the first report on the phytochemical analysis of C. umbrosum which revealed
presence of buddlejasaponin IVa, buddlejasaponin IV, and rosmarinic acid as the main
components of methanol extract [1]. |