چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| AMicroRNA (miR) based electrochemical method for quantification of miR-146a, a known biomarker for neurodegenerative disease, was developed. In this bioassay, the capture microRNA (C-miR)was self-assembled on the gold surface and used for quantification of target microRNA (T-miR) ofmiR-146a. For this purpose, an optimized concentration of C-miR was immobilized on the surface of gold electrode and used for capture of target analyte (T-miR). All of preparation stepswere characterized by electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and atomic force microscopy (AFM). At the optimized conditions, the linear dynamic range, limit of quantification and relative standard deviation of the proposed bioassay were obtained as 10 pM to 1 μM, 10 pM and 1.59%, respectively. The unprocessed human serum sample was used as a real sample and the results fully confirmthat the designedmicroRNA based biosensor is capable for detection of miR-146a as neurodegenerative disease biomarker. The developed method offers a more precise and high sensitive tool to be used in clinical applications for early detection of neurodegenerative disease like Alzheimer's and Parkinson. |
| نویسنده | نفر چندم مقاله |
|---|---|
| بلال خلیل زاده | اول |
| محمدرضا رشیدی شاهگلی | هفتم |
| بهزاد برادران | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| qqq.pdf | 1398/02/30 | 3517788 | دانلود |
