چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| A potential approach for clinical colon cancer therapy is combination chemotherapy. In this study, a biodegradable and pH-responsive nano-carrier was designed for co-delivery of Doxorubicin hydrochloride (DOX) and Hydroxytyrosol (HT) in HT-29 colon cancer cells. For this purpose, Poly (lactide-co-glycolic acid-co-acrylic acid) (PLGA-co-PAA) was synthesized by radical AA telomerization in the presence of mercaptoethanol (ME), followed by the ring-opening polymerization (ROP) of lactide and glycolide in the presence of PAA-OH as a chain transfer agent. Nanoparticles with homogeneous spherical morphology and an average diameter of around 18 nm were obtained; cellular uptake of DOX@HT-loaded nanoparticles was more than 94%. Cell cycle analysis and DAPI staining results revealed a high amount of apoptosis on HT-29 cancer cells treated with the dual drug-loaded nanoparticles in comparison to free drugs and single drug-loaded nano-formulations. RT-PCR analysis indicated that the expression levels of hTERT, CREB1 and CREB2 genes were significantly down-regulated in the presence of nano-encapsulated drugs versus free drugs in the HT-29 cell line. Moreover, results revealed that DOX@HT NPs inhibited gene expression more efficiently than single forms. All these results confirmed that these pHresponsive biodegradable nanoparticles are suitable for combination chemotherapy and further in vivo studies in future. |
| نویسنده | نفر چندم مقاله |
|---|---|
| الهام احمدی | اول |
| نصرت اله ضرغامی | دوم |
| محمد اصغری جعفرآبادی | سوم |
| لیلا علی زاده | چهارم |
| مهران خجسته فرد | پنجم |
| محمد رحمتی | ششم |
| رویا صالحی قره ورن | هفتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| ahmadi paper.pdf | 1398/02/26 | 5023938 | دانلود |
