چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| هادی قنبری | اول |
| محمد یوسف معمار | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Grape Seed Effect, Gram negative bacteria, Biofilms |
| خلاصه مقاله | Biofilm producer Gram negative bacteria are the major public health problem. In vitro studies have described that the microbial biofilms are significantly more resistant to antimicrobial agents than their planktonic growth forms [1]. Current Investigation was performed to study the antibiofilm effect of Grape seed extract (GSE) on biofilm forming Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii isolates. Thirty bacterial isolates were collected from clinical specimens. The bacterial identification was performed by the standard microbiological methods. The antimicrobial resistance pattern was determined by the Disk diffusion agar. After GSE extraction, the total poly phenolic content of GSE was detected by Folin Ciocalteu method. Detection of biofilm formation was performed by the microplate assay and crystal violet dye. For study of antibiofilm activity of GSE, biofilm inhibitory concentration (BIC) were detected previously described method. Different incidence of resistance was observed to different antibiotics. Mean BIC of GSE were 2000µg/mL for Enterobacteriaceae and 4000µg/mL for Pseudomonas aeruginosa after 72h incubation. GSE did not show detectable antibiofilm effects after 12h and 24h. A significant antibiofilm activity was not observed agonist Acinetobacter baumannii. The significant antibiofilm effects of GSE agonist Enterobacteriaceae and Pseudomonas aeruginosa make it a promising option for the development a new strategy for treatment of biofilm related infections. However, further studies should be clarified to elements in the GSE due to the special pharmacodynamic effect. The association between the antibiofilm activity of the GSE with the specific bacterial targets should be more investigate and the primary mechanisms of antibacterial activity, synergies and antagonisms effects with others antimicrobial component should be studied. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
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| New Doc 2019-05-08 12.01.34_3.jpg | 1398/02/18 | 153428 | دانلود |
| New Doc 2019-05-08 12.01.34_6.jpg | 1398/02/18 | 387216 | دانلود |
| New Doc 2019-05-08 12.01.34_4.jpg | 1398/02/18 | 71569 | دانلود |
| New Doc 2019-05-08 12.01.34_7.jpg | 1398/02/18 | 217209 | دانلود |
| poster ghanbari.pdf | 1398/02/18 | 297270 | دانلود |
| New Doc 2019-05-08 12.01.34_5.jpg | 1398/02/18 | 311344 | دانلود |
| New Doc 2019-05-08 12.01.34_8.jpg | 1398/02/18 | 181213 | دانلود |
| 1.docx | 1398/02/24 | 22654 | دانلود |
| 2.docx | 1398/02/24 | 22180 | دانلود |
| 1.docx | 1398/02/24 | 14160 | دانلود |
| 2.docx | 1398/02/24 | 15824 | دانلود |
