| The most commonly used methods for the preconcentration of metal ions are expensive or need many expensive instruments. However, spectrophotomtric methods have recently attracted considerable attention owing to their rapidity, simplicity, economy, and good separation yields. In this study a new and extraction simple method was developed for the preconcentration and spectrophotometric determination of Co (II) in aqueous phase using cloud point extraction (CPE). This technique is based on the complexometric reaction of Co (II) with dithizone (DZ) as a chelating agent and sodium dodecyl sulfate (SDS) as an extracting agent at room temperature. The parameters affecting the CPE including pH, NaCl, DZ concentration, and SDS concentration were optimized. Besides, the interference effect of some anions and cations was evaluated. Overall, DZ gave the most desirable property in terms of rapid extraction and separation of Co (II), on all tested conditions. Finally, the method was successfully used for the determination of Co (II) in vitamin B12 ampoule. This study demonstrates that cloud point extraction may offer a promising technique for the preconcentration of metal ions in water samples. |