تاثیر توام سربرولایزین و محیط غنی شده بر رفتار شبه اضطرابی و نقص حافظه ناشی از مدل اختلال استرس پس از سانحه در موش کوچک آزمایشگاهی

مجریان: جواد محمودی

خلاصه روش اجرا: In this study, 50 male C57 mice in the weight range of 28-30 g will be used. Animals will be divided into following groups (n=10 each): 1- Control 2- NS 3- CBL 4- EE 5- CBL+EE Animals in control group will receive intraperitoneal (i.p.) NS (2.5 ml/kg) for 15 consecutive days. In other remaining groups, following induction of the PTSD protocol, each group will receive their respective treatments for 15 consecutive days as below, Animals in NS group will receive i.p. NS (2.5 ml/kg). CBL mice will receive i.p. injection of 2.5 ml/kg CBL. Mice in EE group will be kept in the EE cage. In CBL+ EE group, mice will be kept in EE cage and injected with 2.5 ml/kg i.p. CBL. After this 15-days period, animals will be assessed by lashlly and hole board tests for evaluation spatial and anxiety-like behaviors, respectively. PTSD procedure Electric foot shocks will be delivered through the grid floor using an isolated shock generator. Each mouse will be placed in the chamber, and after a 5-min adaptation period, a total of 15 intermittent, inescapable foot shocks (intensity: 0.9 mA, interval: 8 s, and duration: 10 s) will be delivered for two consecutive days (11). Control animals will be underwent the same condition as mentioned above except that, they will not receive any aversive foot shock. Enriched Environment (EE) EE will be prepared by a big box equipped with components including running wheel, colorful balls and tunnel as well as round beads wire maze, marble run plastic swing rack, mice igloo as well as pellet food inside plastic feeding dish. Two water bottles have been mounted in lateral walls of the EE chamber to provide unlimited access to water for mice inside it. 24 h, after the end of behavioral tests, blood samples will be taken to assess the level of corticosterone and hippocampal samples will be collected to evaluate the oxidative stress by colorimetric methods and expression of synaptic proteins including GAP-43, SYP, PSD-95 by blotting method.