A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis
A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis
نویسندگان: محمد حسن زاده محله , ابوالقاسم جویبان , جعفر سلیمانی
کلمات کلیدی: Biomedical analysis, Glycoprotein, Immunoassay, Conductive ink, Biomarker, Nanomaterials, Lab on paper
نشریه: 24084 , 01 , 146 , 2019
| نویسنده ثبت کننده مقاله |
محمد حسن زاده محله |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
مرکز تحقیقات کاربردی دارویی |
| کد مقاله |
65647 |
| عنوان فارسی مقاله |
A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis |
| عنوان لاتین مقاله |
A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis |
| ناشر |
6 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
خیر |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
|
| The technology of ink-jet printing has been an appealing alternative to traditional photolithography for fabricating flexible and elastic circuits, having low-cost and ease of control. In this work, a novel, unique and sensitive immunoassay designed based on application of silver nanoparticles-reduced graphene oxide nanocomposite (Ag/RGO) ink for fabrication of paper based immunosensor, Cysteamine coped gold nanoparticles (CysA/Au NPs) as a signal amplification element for determination of CA15-3 protein. Fully electrochemically methodology was applied to prepare a new transducer based on the Ag/RGO surface which provides high surface area for CA15-3 antibody immobilization in high amount. Under optimized experimental conditions, the Ag-RGO/CysA-Au NPs based immunosensor shows good specificity and sensitivity. The obtained LLOQ of immunosensor for CA 15-3 biomarker was 15 U/mL as well as calibration curve was linear in the range of 15–125 U/mL. The technique was successfully employed assay of CA15-3 biomarker in unprocessed human plasma samples. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| 176.pdf | 1397/10/27 | 4506726 | دانلود |
