اندازه گیری تعدادی از ترکیبات فسفولیپیدی در نمونه های بازدم نوزدان نارس تحت درمان با سورفکتانت های ریوی

مجریان: مریم خوب نسب جعفری

خلاصه روش اجرا: For this purpose, firstly 2 mL of the collected liquid in the expiratory circuit of the mechanical ventilators and it is transferred into a glass test tube. Then a few microliters of prepared deep eutectic solvent composed of phosphocholine chloride (PChCl) and p-aminophenol (AmP) is added to the tube as extraction solvent and agent and it is sucked into a glass syringe and pushed out to the tube. In this step, the analytes are extracted into the fine droplets of PChCl: AmP. The cloudy solution is centrifuged and the whole of the sedimented phase is injected into the HPLC-DAD system for analysis. All of the affective parameters on the performance of the method like extraction solvent kind, the sample solution pH, ionic strength, and dispersive solvent volume is optimized with response surface method (RSM). Under optimal conditions, the figure of merits of the method including limit of detection, limit of quantification, lower limit of quantification, linear range, selectivity, accuracy, extraction recovery, stability, repeatability, and enrichment factor are evaluated according to Food and Drug Administration Guidance.