چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Recurrent pregnancy loss (RPL) is a multifactorial disorder of women in reproductive age, which in some cases is caused by immunologic abnormalities. In this study, we aimed to evaluate cellular and molecular components of the immune system like different T‐cell subsets and their regulating microRNAs (miRNAs) in RPL women and control group. Fifty RPL and 50 healthy subjects were recruited. Subsets of T cells, including regulatory T (Treg) cells, helper T (Th) 17 cells, exhausted T cells, exhausted Treg cells were evaluated by flow cytometry. Transcription factors of T cells and related miRNA profile were quantified using real‐time polymerase chain reaction (RT‐PCR). Assessment showed that Treg and exhausted T cells, were decreased in RPL patients (p = 0.009 and 0.02, respectively), while an increase was observed in Th17 and exhausted Treg frequency (p = 0.013 and 0.0037, respectively). Messenger RNA expression level of T‐bet and IRF4 was upregulated in RPL patients (p = 0.011 and 0.0001, respectively), while Th2‐ and Treg‐ related transcription factors, GATA3 and GITR, were downregulated in these patients compared with the healthy subjects (p = 0.0008 and <0.000, respectively). Treg‐associated miRNAs, the miR‐106b‐25–93 cluster, showed a higher rate in RPL patients (P = 0.007, 0.001, and 0.029, respectively), however, we observed no significant difference in the expression level of Th17‐associated miRNA, mir‐326. According to the results, we concluded that unbalanced immune responses and deregulated function of T‐cell subsets may lead to reproduction‐related failure like a miscarriage. Therefore, evaluation of immune cells and related miRNA profile may serve as prognostic biomarker for the treatment of RPL patients. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سمانه عبدالمحمدی وحید | اول |
| مهناز غائبی | دوم |
| مجید احمدی | سوم |
| محمد نوری | چهارم |
| شهلا دانایی | پنجم |
| لیلی عاقبتی | ششم |
| رضا موسوی اردهائی | هفتم |
| بهمن یوسفی | هشتم |
| پروین حکیمی | نهم |
| رضا ریخته گر غیاثی | یازدهم |
| مهدی یوسفی | دوازدهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Altered Tcell subpopulations in recurrent pregnancy loss.pdf | 1397/10/14 | 958151 | دانلود |
