چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مهدی احمدی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 65496 |
| عنوان فارسی مقاله | مقایسه تزریق موضعی و سیستمیک ترشحات سلولهای بنیادی بر کاهش تغییرات آسماتیک القا شده توسط اووالبومین در رتها |
| عنوان لاتین مقاله | Comparison between local and systemic administration of MSC-derived CM on ameliorating OVA-inducted asthmatic changes in rats |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | دومین کنگره بین المللی زیست پزشکی ایران |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1397 |
| سال انتشار/ارائه میلادی | 2018 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1397/10/03 الی 1397/10/06 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.icb2018.com/fa/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهدی احمدی | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction: The term asthma is devoted to life-threating chronic changes in respiratory system with extensive burden on socioeconomic costs, involving approximately 300 million of the world population. In spite of tremendous advances in human medicine, asthma cannot be cured permanently yet. Although, routine medications is considered as the cornerstone of treatment strategy, but they have many unwanted side effects. Of note, new strategic approaches seem to be essential for facilitating the regeneration rate of any structural changes in the asthmatic airways. OVA with aluminum hydroxide, as an adjuvant, is one of the most common antigen used in inducing asthmatic changes in animal models and it is a suitable choice for the development of Th2-type allergic inflammatory response. Today, Stem cell therapy has opened a promising therapeutic strategy for asthma. Selecting MSC-derived conditioned media (CM) instead of stem cells, could circumvents some of the pitfalls of MScssuch asrisk of developing cancer, xenozootic contamination sand total costs. Our objective was to compare the potential therapeutic effects of MSC-derived CM after either local orsystemic administration on ameliorating OVA-inducted asthmatic changes. Materials and methods: 28 male Wistar rats(200-250 g, 6–8 weeks old) wereenrolled to the current study.Fourhealthy rats were blindly used for isolationof rat bone marrow-derived MSCs (rBMMSCs) and Conditioned Media (CM) harvesting.The rest of 24 animalswere classified into four experimental groups (n=6 for each group); control rats (C group),Sensitized rats (S group), Sensitized rats received intratracheally50 μlCM (ST+CM group) and Sensitized rats received intravenously 50 μlCM (SV+CM group).Rats in the sensitized groupswere actively exposed to OVA over a period of 32 ± 1 days. In the control group, normal saline solution was applied instead of OVA.One-day post sensitization(day 33), SV+CM group and ST+CM group received CM intratracheally and intravenously, respectively. Two weeks post treatment with CM (day 45);the expression of interleukin (IL)-4, IL-13, and IL-10in lung tissues of asthmatic rats was measured by real-time PCR. Results: Our data indicated a marked expression in the levels of IL-4 and IL-13 with decrease of IL-10 in asthmatic groups as compared to healthy rats (p < 0.001 to p < 0.01). The expression of measured mRNA in ST+CM groupshowed drastic difference compared with other sensitized groups. In detail, the expression levels of IL-4 and IL-13 diminished significantly (p<0.01), whereas IL-10 increased simultaneously (p<0.05). In addition, the pattern of IL4, IL-13 and IL-10 expression in SV+CM group were more similar to group S (Figs a, b, c). Conclusion: Histopathological changes in the asthmatic lung tissues are mainly induced by the Promotion of airway inflammation due to the shift in the Th1/Th2 balance toward a Th2-dominant phenotype. Regarding to asthma pathology monitoring of Th1 and Th2 cytokine levels is crucial.The results of this study showed directadministration of CM could be effective in alleviation of asthmatic changes. However, no significant modulatory effects originating from systemic administration of CM were also evident in asthmatic rats. The CM content and existence of modulatory factors must be detected in the future investigations. |
| کلمات کلیدی | Asthma,MSC-derived CM, Intravenously, Intratracheally |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| certifect3 001.jpg | 1397/11/03 | 545378 | دانلود |
| certifect2 001.jpg | 1397/11/03 | 405203 | دانلود |
| 1.pdf | 1397/11/03 | 335815 | دانلود |
