چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| رضا رهبرقاضی | پنجم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim: Extracellular vesicles (EVs) are nano-sized vesicles released by many cells as intercellular communication tools. Recent studies have shown that diabetes mellitus (DM) affects the biology of stem cells especially mesenchymal stem cells (hMSCs) through changes in their secretome. We aimed to study the effect of serum from type 2 diabetic mellitus patients on the size and Zeta potential (ZP) of hMSCsderived EVs in vitro. Methods: hMSCs were allocated into two groups as follows; Control (cells received DMEM/LG and 10% sera from healthy subjects) and Diabetic (cells received DMEM/LG and 10% diabetic sera). Treatment was carried out for a period of 7 days. EVs were then isolated according to the isolation kit (Publication No: MAN0006949, Invitrogen) instructions. Flow cytometry and scanning electron microscopy were used to characterize the EVs. To analyze size distribution and ZP, EVs were subjected to the Zetasizer Nano Z system (Nano ZS ZEN 3600, Malvern). Furthermore, to determine whether the DM sera could affect the dynamic of the fatty acids profile, we used Gas Chromatography (GC). Data are expressed as means ± SD. Student’s t-test was used to calculate the significance of differences between the groups. Values of P<0.05 were considered statistically significant. Results: The size of the EVs derived from the diabetic group significantly increased compared to the control group (p control versus diabetic <0.05). Furthermore, in comparison with the control group, the ZP of diabetic hMSCs-derived EVs significantly decreased (p control versus diabetic <0.05). Data from GC showed that treatment with DM sera resulted in a highly significant increase in the content of Palmitate (16:0) (p control versus diabetic <0.01) and a significant decrease in the content of Myristate (14:0) and Linoleate (18:2) compared to the control group (p control versus diabetic <0.05).No significant differences in the content of other fatty acids between the control and diabetic groups were observed (p control versus diabetic >0.05). Conclusion: We conclude that diabetic mellitus alters the properties of EVs and the fatty acid profile leading to modifications in the cell biology of hMSCs. |
| کلمات کلیدی | Extracellular vesicles; Mesenchymal stem cell; Diabetes mellitus; Zeta potential |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| image.jpg | 1397/10/09 | 359866 | دانلود |
| Diabetic Human.pdf | 1397/10/09 | 227872 | دانلود |
