چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| سپیده ششپری | اول |
| مهناز شهنازی منصوری | دوم |
| شاهین احمدیان | سوم |
| محمد نوری | چهارم |
| مهران مسگری عباسی | پنجم |
| رضا رهبرقاضی | هفتم |
| مهدی مهدی پور | هشتم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim: Today Cell therapy is one of the new therapeutic methods in medical sciences and fertility related complications such as menopause. Bone marrow derived c-kit positive cells, have the ability to differentiate into different cell types. In this study, intra-ovarian injection of bone marrow derived c-kit positive cells with the aim of ovarian rejuvenation was implemented in the rat model of ovarian failure. Methods: To induce the rat model of menopause, daily injection of 4-vinylcyclohexene dioxide (VCD) was performed for 15 days. 75 Wistar female rats were randomly divided into 5 groups: Control (IP injection of saline for VCD control), VCD free (IP injection of VCD and letting them free without intervention), Ckit positive (IP injection of VCD and intraovarian injection of Ckit positive cells), Ckit negative (IP injection of VCD and intra-ovarian injection of Ckit negative), saline injection (IP injection of VCD and intra-ovarian injection of saline). Histological evaluation and hormonal assay (FSH and E2) continued up to 8 weeks (once every 2 weeks). At the end stage, reproduction performance of 3 rats for each group was assessed. Results: Preliminary results from histological evaluation showed an improved follicles counts in the c-kit positive group compared to c-kit negative and saline groups, whereas more atretic follicles in different stages were noticed in c-kit negative rats and saline group. Reproductive performance assessment after mating revealed a significantly higher birth rates in c-kit positive group compared to c-kit negative, saline and VCDfree groups. Conclusion: Intra-ovarian injection of Ckit positive derived from bone marrow cells is a useful approach for ovarian tissue rejuvenation and reproductive performance return. Future studies and clinical trials will hopefully focus on the uses of Ckit positive cells in the restoration of human ovaries. |
| کلمات کلیدی | Infertility; Menopause; CKit positive; Bone marrow cells; Ovarian rejuvenation |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| B1.png | 1397/10/08 | 190434 | دانلود |
| B2.png | 1397/10/08 | 187109 | دانلود |
| x1.jpg | 1397/10/08 | 432235 | دانلود |
