چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | رضا رهبرقاضی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز سلولهای بنیادی |
| کد مقاله | 65472 |
| عنوان فارسی مقاله | Effect of Rat Cardiomyoblast Encapsulation with Alginate-Gelatin Microspheres on Cell Dynamic and Viability |
| عنوان لاتین مقاله | Effect of Rat Cardiomyoblast Encapsulation with Alginate- Gelatin Microspheres on Cell Dynamic and Viability |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | سومین جشنواره ملی و کنگره بین المللی علوم و فناوری سلول های بنیادی و پزشکی بازساختی |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1397 |
| سال انتشار/ارائه میلادی | 2018 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1397/09/07 الی 1397/10/10 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://stemcellfestival.com/Default.aspx?PN=296 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| حسن امینی | اول |
| مینا ممی پور | دوم |
| محمد رضا یوسفی | سوم |
| شهریار هاشم زاده | چهاردهم |
| محمد نوری | هفتم |
| شیرین صابریان پور | هشتم |
| رضا رهبرقاضی | نهم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Rat cardiomyoblasts; Encapsulation; Alginate-gelatin Microspheres; Oxidative stress enzymes; Cell Viability |
| خلاصه مقاله | Background and Aim: Despite the emergence of novel therapeutic advances regarding MI, ischemic heart disease is conceived as a major cause of global mortality and morbidity. it made investigators to develop novel re-vascularization techniques that specifically target the microvasculature in ischemic myocardium. Several studies have recently reported promising results by modifying and enhancing cell-mediated ischemic myocardial repair and regeneration. In this study, we aimed to investigate the effect of alginate-gelatin encapsulation on the viability and dynamic of rat cardiomyoblasts in vitro. Methods: Rat cardiomyoblasts cell line H9C2 were encapsulated by alginate-gelatin solution and incubated for 7 days, and the control group was rat cardiomyoblasts from the same cell line without encapsulation while incubated for 7 days as like the encapsulated group. The level of extracellular enzymes as like SGPT, SGOT, CPK, and LDH were detected in supernatants from both groups. The level of intracellular enzymes as like SOD, GPx, and TAC was measured by biochemical analyses in cell lysates. To the examination of cell viability, we used MTT method. Results: We found that encapsulation was able to increase the viability of rat cardiomyocytes after 7 days. The increased level of intracellular enzymes including SOD, GPx, and TAC was identified compared to the control cells (P>0.05). No significant differences were found regarding the level of SGPT and CPK while the level of LDH and SGOT as extracellular enzymes showed decreasing compared to the control nontreated cells (P>0.05). Conclusion: The results of this investigation showed that encapsulation of rat cardiomyoblasts with alginate-gelatin microspheres improves the cell's dynamic and viability. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Amini.pdf | 1397/10/07 | 179254 | دانلود |
| 53522F32-B25A-498D-A4CF-A358152EF00A.jpeg | 1397/10/11 | 944224 | دانلود |
