چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | رضا رهبرقاضی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز سلولهای بنیادی |
| کد مقاله | 65342 |
| عنوان فارسی مقاله | Alginate-Gelatin Encapsulation of Endothelial Cells Induces Angiogenesis In In Vivo and In Vitro Milieu |
| عنوان لاتین مقاله | Alginate-Gelatin Encapsulation of Endothelial Cells Induces Angiogenesis In In Vivo and In Vitro Milieu |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | سومین جشنواره ملی و کنگره بین المللی علوم و فناوری سلول های بنیادی و پزشکی بازساختی |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1397 |
| سال انتشار/ارائه میلادی | 2018 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1397/09/07 الی 1397/09/10 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://stemcellfestival.com/PrintAbs.aspx?EID=311 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| آیسا رضابخش | دوم |
| علیرضا نورآذریان | چهارم |
| مجید خاکسار | هفتم |
| رضا رهبرقاضی | یازدهم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim : Up to present, numerous attempts have been collected in the favor of cell-based therapies by using sophisticated methodologies and delivery approaches. Micro-capsules have potential to provide safe micro-environment for cells delivery during transplantation in a physiological 3D milieu. Methods : Here, we investigate the impact of alginate gelatin encapsulation on angiogenic potential of human endothelial cells after 5 days. Human umbilical vein endothelial cells were encapsulated by alginate-gelatin substrate and kept in vitro for 5 days. Cell survival assay (MTT) and autophagy PCR array analysis were used to study HUVECs survival rate. To monitor angiogenesis capacity, cell distribution of Tie-1, VEGFR-1, and VEGFR-2 and Tie-2, were measured by ELISA. In addition to in vitro tubulogenesis assay, we measured the protein level of VE-cadherin by Western blotting. The migration of encapsulated HUVECs was confirmed by measuring MMP-2 and MMP-9 activity via gelatin zymography. The in vivo angiogenic potential of encapsulated HUVECs was analyzed in immune-compromised mouse implant model during 7 days post-transplantation. Results : Encapsulation increased HUVECs cell survival and proliferation rate. Compared to the control, no significant differences were observed in autophagic response of encapsulated cells (p > 0.05). The cell distribution of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 were induced, but did not reach to significant levels. Encapsulation suppressed MMP-2, -9 activities while increased the VE-cadherin peptide in enclosed cells (p < 0.05). Moreover, an enhanced in vivo angiogenic response of encapsulated HUVECs was evident as compared to non-capsulated cells (p < 0.05). Conclusion : Data suggest that alginate-gelatin encapsulation induces angiogenic response of endothelial lineage in in vivo and in vitro conditions. |
| کلمات کلیدی | alginate-gelatin encapsulation, angiogenesis, human endothelial cells, migration, receptor tyrosine kinases, VE-cadherin |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 111.jpg | 1397/09/23 | 115065 | دانلود |
| 2222.jpg | 1397/09/23 | 78751 | دانلود |
| 7f720c5d-2c13-4ce1-b1fa-375ae2b3b64b.JPG | 1397/09/23 | 125392 | دانلود |
| IMG_3368.jpg | 1397/09/26 | 608107 | دانلود |
