چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مسعود دارابی امین |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز سلولهای بنیادی |
| کد مقاله | 65289 |
| عنوان فارسی مقاله | The Effect of ROCK Inhibitor on Recovery and Proliferation of Thawed Pluripotent Stem Cells |
| عنوان لاتین مقاله | The Effect of ROCK Inhibitor on Recovery and Proliferation of Thawed Pluripotent Stem Cells |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | سومین جشنواره ملی و کنگره بین المللی علوم و فناوریهای سلولهای بنیادی و پزشکی بازساختی |
| نوع کنگره / همایش | ملی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1397 |
| سال انتشار/ارائه میلادی | 2018 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1397/09/07 الی 1397/09/10 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://stemcellfestival.com/Default.aspx?PN=296 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| اشکان کلانتری چروده | اول |
| سید وحید حسینی | دوم |
| مسعود دارابی امین | چهارم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Pyridines, embryonic stem cells, induced pluripotent stem cells, cryopreservation |
| خلاصه مقاله | Background and Aim: Both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have significant obstacles for culture with inactivated feeder layer when defreezed from cryopreserved stocks. ROCK inhibitor treatment enhances stem cell survival efficiency and poor recovery after thawing. In this study, we investigated the effect of different incubation times of a specific pyridine-based Rho kinase (ROCK) inhibitor on recovery enhancement and proliferation of thawed hESCs and iPSCs. Methods: The protocol of this study was approved by the local ethics committee (code IR.TBZMED.REC.1396.1031). Mouse embryonic fibroblasts (MEFs) were cultured in DMEM supplemented with 10% FBS. Before use as a feeder layer, their growth was arrested with Mitomycin C (2 mg/ml) for 3 hours in 37°C incubator. Inactivated cells were seeded at 600,000 cell density on 6 cm culture plates. Pluripotent stem cell medium containing DMEM/F-12 and Knockout Serum Replacement was conditioned for 4 hours on inactivated MEFs. Cryovials of Royan H6 hESC and R1 hiPSC4 lines were thawed immediately into conditioned media with Y-27632 ROCK inhibitor at 10 μM concentration. To investigate the effect of Y-27632, medium was refreshed with basic medium without inhibitor at different time points after 8-24 hours. The number of colonies and cell proliferation were assessed microscopically. Results: We observed that the number of adherent colonies was significantly higher when the medium supplemented with Y-27632 removed after 8-12 h from culture plates (≥2-fold versus later time points). In addition, the rate of reaching confluency after 10 days was higher in the mentioned culture condition (80% versus <60% at later time points), which was more prominent in iPSCs. But when we removed ROCK inhibitor after 18 or 24 hours, both hESCs and iPSCs reached to confluency later, after 12 days. Conclusion: We demonstrated that a short time treatment with ROCK inhibitor only for 8-12 hours is more appropriate for recovery and proliferation of thawed pluripotent stem cells, maybe due to the inhibitory effect of ROCK inhibitor on cell proliferation in long-term incubation. According to our results optimizing of pluripotent stem cell culture condition with respect to ROCK inhibitor treatment time is essential. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| congress abstract.pdf | 1397/09/18 | 124176 | دانلود |
| New Doc 2018-12-09 18.34.11_1.jpg | 1397/09/18 | 1150691 | دانلود |
| PS-102.pdf | 1397/09/18 | 808177 | دانلود |
| article collection of congress.pdf | 1397/09/18 | 3845453 | دانلود |
