چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | محمد نوری |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز جامع سلولهای بنیادی و پزشکی بازساختی SCARM |
| کد مقاله | 65266 |
| عنوان فارسی مقاله | The potential of human amniotic membrane proteins in cardiac regeneration; the role of extraction methods |
| عنوان لاتین مقاله | The potential of human amniotic membrane proteins in cardiac regeneration; the role of extraction methods |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | The Second National Festival&International congress on Stem Cell & Regenerative Medicine |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1396 |
| سال انتشار/ارائه میلادی | 2017 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1396/04/22 الی 1396/04/24 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://bit.ly/2zR5EgD |
| آدرس علمی (Affiliation) نویسنده متقاضی | Stem Cell And Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سمیرا نوزری | دوم |
| محمد نوری | سوم |
| یوسف فریدوند | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction and objectives: The strategy to induce proliferation and cardio-protection against cardiac damage is one of the main approaches for inhibition of cardiac complication in heart disease. In this current study, we aimed to assess the proliferative activity of human amniotic membrane extracted proteins (HAM-EPs) by three different extraction methods from HAM derived from human placenta in H9C2, a cardiomyocyte cell line. Methods: The rat cardiomyocyte cell line, H9c2, was cultured with DMEM/HG medium, supplemented with 10% FBS and antibiotics. Placenta was obtained from healthy women after normal or caesarean delivery . After removing HAM, three methods were used for protein extraction . First, HAM was chopped, sonicated and homogenized. Then, proteins were collected after 10,000g centrifuge in PBS buffer. In the second method, HAM was homogenized at the RIPA buffer and after being dialyzed against 10 mM PBS pH 7.4, the proteins were collected after 10,000g for 30min at 4°C. In the third method, HAM was minced and grinding into powder using liquid nitrogen and homogenized into PBS buffer. After characterizing the extracted proteins by SDS-PAGE, H9c2 cells were exposed to 1 mg/ml and 0.5 mg/ml of HAM-EPs. The effects were evaluated by using the MTT assay test for 24, 48 and 72 h, Ki-67 staining and flow-cytometry with Annexin V/PI Results: The results indicated that treatment with 1 mg/ml of HAM-EPs induced the cell proliferation but higher concentrations were accompanied with cell toxicity which observed in MTT assay results. The incubation with protein extracted by PBS method showed the highest number of cell proliferation compared with the RIPA and liquid nitrogen methods. Also, increase in Ki-67 expression was observed in PBS extraction method which is in line with Annexin V/PI staining Conclusion: Taken together, the study results demonstrated that HAM-EPs exert proliferative effect on H9C2 cell line. This proliferation potency was significantly depended on methods of preparing extracted protein |
| کلمات کلیدی | Human amniotic membrane, Protein extraction, Cardiomyocyte, H9C2 |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| page 154-155 issue 15.pdf | 1397/09/17 | 1783470 | دانلود |
| certificate.jpg | 1397/09/19 | 1486345 | دانلود |
