چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Despite at the beginning known as a benign disease, endometriosis is defined as a risk factor for developing ovarian carcinoma. Nowadays, the effect of endometriosis on ovarian malignancy is known but its role in granulosa cell tumor is still unclear. Methods and Materials: In this study serum samples were collected from patients with endometriosis and divided into whole and steroid depleted groups. Desertification was performed according to the charcoal-dextran protocol and the sera were added to the culture media of granulosa cells retrieved from tubal or male factor infertile women. qRT-PCR and flowcytometry was performed to determine the expression level of inflammatory and apoptotic genes and apoptosis level of granulose cells respectively. Total concentration of lipid was measured using gas chromatography method in the granulosa cells. Results: Results revealed that the expression of AKT and NF-κB/RelA genes were significantly higher in the granulosa cells treated with steroid depleted serum obtained from patients with distrificated endometriosis (DE) compared to the control group (9.39 and 7.9 folds respectively. p<0.0001). In the DE group, the decline pattern of expression was observed for the genes related to apoptosis. The synthesis of saturated was significantly decreased and unsaturated fatty acids was increased in DE group, respectively. Conclusion: The effect of steroids on endometriosis is contradictory. The level of cortisol and sex hormones could be affected by endometriosis, causing alterations of the disease progression. Reduced level of steroid hormones in patients with endometriosis may be considered as a critical risk factor for granulosa cell tumor. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سمیرا اصغری | اول |
| امیر ولیزاده دیزجیکان | دوم |
| مجید احمدی | سوم |
| ابوالفضل برزگری | چهارم |
| رضا ریخته گر غیاثی | پنجم |
| صنم دولتی | ششم |
| شهلا دانایی | هفتم |
| صدیقه عبدالهی فرد | هفتم |
| محمد نوری | هشتم |
| مهدی مهدی پور | نهم |
| مهدی یوسفی | دهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 10.1002@jcp.27862.pdf | 1397/09/17 | 647307 | دانلود |
