چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Vascular endothelial growth factor receptor 2 (VEGFR2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR2 called Kinase insert domain-containing receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified. |
| نویسنده | نفر چندم مقاله |
|---|---|
| جلال عبدالعلیزاده | ششم |
| محمد رحمتی | دوم |
| ابوالفضل برزگری | پنجم |
| مهدی اصغری وسطی کلایی | سوم |
| شیرافکن کردی | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment.pdf | 1398/03/18 | 857753 | دانلود |
