چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Quercetin, a flavonoid antioxidant, has been found to exert therapeutic effects in diabetic condition. Autophagy represents a homeostatic cellular mechanism for the turnover of unfolds proteins and damaged organelles through a lysosome-dependent degradation manner. We speculated that quercetin could protect endothelial cells against high glucose-induced damage by promoting autophagic responses. Methods: HUVECs viability was evaluated by MTT method. Griess and TBARS assays were used to monitor the levels of NO and MDA, respectively. Intracellular ROS generation was determined in DCFDA-stained cells analyzed by flow cytometry. To investigate the role of quercetin in endothelial cell migratory behavior, we used a scratch test. The level of autophagy proteins LC3, Beclin-1 and P62 were measured by western blotting technique. Results: Our results showed that quercetin had the potential to increase cell survival after exposure to high glucose (P<0.05). Total levels of oxidative stress markers were profoundly decreased and the activity of GSH was increased by quercetin (P<0.05). High glucose suppressed HUVECs migration to the scratched area (P<0.05). However, a significant stimulation in cell migration was observed after exposure to quercetin (P<0.05). Based on data, autophagy was blocked at the late stage by high glucose concentration while quercetin enhanced autophagic response by reducing the P62 level coincided with the induction of Beclin-1 and LC3-II to LC3-I ratio (P<0.05). All these beneficial effects were reversed by 3-methyladenine as an autophagy inhibitor. Conclusion: Together, our data suggest that quercetin could protect HUVECs from high glucose induced-damage possibly by activation of the autophagy response. |
| نویسنده | نفر چندم مقاله |
|---|---|
| آیسا رضابخش | اول |
| رضا رهبرقاضی | دوم |
| فرزانه فتحی | چهارم |
| آزاده منتصری | پنجم |
| علیرضا گرجانی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Aysa10.1016@j.phymed.2018.11.008.pdf | 1397/08/26 | 5595314 | دانلود |
