چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| The study describes the fabrication of an implantable nanofibrous scaffold loaded with 17AAG as a heat shock protein 90 (Hsp90) inhibitor for possible application in breast cancer therapy. In order to, 17AAG loaded PCL/PEG nanofibers (NFs) were prepared by electrospinning technique and their chemical and physical properties studied by FTIR and FESEM. To assess cytotoxicity of 17AAG loaded PCL/PEG NFs and free 17AAG on T47D cancer cells as in vitro model of breast cancer, MTT assay was performed. The mRNA expression level of Hsp90 and telomerase activity in treated cells with 17AAG loaded PCL/PEG NFs also were investigated using real-time PCR (RT-PCR) and TRAP method, respectively. MTT method indicated which loading of the 17AAG into PCL/PEG NFs increased effectively cytotoxicity against T47D cells. This result was correlated with significant decrease of telomerase activity and mRNA expression level in treated cells with 17AAG loaded PCL/PEG NFs compared to free 17AAG. In conclusion, the results confirmed which 17AAG loaded PCL/PEG NFs are further effective compared with free 17AAG on T47D breast cancer cells through reduction of telomerase activity and Hsp90 mRNA expression level. Therefore, 17AAG loaded NFs might be a superior device to remove of residual breast cancerous cells and prevent locally cancer recurrence. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سونا طلایی کهجوق | اول |
| حسن ملت یار کوزه کنان | دوم |
| یونس پیله ور سلطان احمدی | سوم |
| ابوالفضل اکبرزاده | پنجم |
| نصرت اله ضرغامی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| talaie1.pdf | 1397/08/26 | 2530210 | دانلود |
| 17-Allylamino-17-demethoxygeldanamycin loaded PCLPEG.pdf | 1397/09/17 | 1173306 | دانلود |
