چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Scrophularia umbrosa is a plant used as a traditional herb. Objectives: This study was designed to investigate the antimalarial activity and free radical scavenging of methanol, DCM and nHexane extract of aerial parts and seed, as well as total phenol, total flavonoid content, and phytochemical identification. Methods: GC-MS techniques were used for identification of the chemical composition of the DCM and n-Hexane extract as well as HPLC and NMR techniques for methanolic extract. The hemozoin formation has been suggested as a desirable drug target to antimalaria sieve scheme. In the present study, we used the spectrophotometric assay to screen plant extracts. The DPPH method has been used for antioxidant activity screening of the extracts and fractions. AlCl3 and Folin -Ciocalteu reagents, respectively, were used for total flavonoid and total phenol content of the samples. Results: Our results showed that n-hexane and the MeOH extracts, aerial part, and seed did not display any impressive prevention of heme biocrystallization confidants, however, the DCM extract of aerial part, in comparison with the blank, showed slightly antimalarial effects. Furthermore, fractions (20%, 40%, and 60%) of MeOH extracts of aerial part and seed of S. umbrosa had moderate to strong antioxidant activity and it is possible to conclude that there is a positive relation between phenolic and flavonoid composition as well as antioxidant activity. GC-MS data showed that volatile portions of DCM and n-Hexane extracts of various organs from S. umbrosa are containing few identifiable compounds. In addition, Aucubin and Luteolin-7-O-rutinoside were also recognized from MeOH extracts. Conclusions: Based on the results, the fractions of extracts could be evaluated by 1HNMR for predicting of groups of natural compounds as well as interfacing chemical and biological assessments. |
| نویسنده | نفر چندم مقاله |
|---|---|
| فریبا حشمتی افشار | دوم |
| الهامه نیکخواه | اول |
| حسین بابائی | سوم |
| عباس دل آذر | چهارم |
| پرینا اصغریان | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 2018-131.pdf | 1397/09/19 | 241883 | دانلود |
