چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Introduction: There are evidences that oxidative stressplay an important role in male infertility. The aim of this study is to investigate the effect of BSO-induced oxidative stress on ultrastructure of testis and sperm fertility in mice. Methods: In this study 30 adult male and 10 adult female mice are used. Male mice were divided into3 groups. In control group, mice did not receive any chemical. In experimental group, mice received 2mmol/kg BSO, 35 days, IP injection. In sham group, mice received solvent of BSO (0.9% saline). After BSO treatment, mice in 3 groups were sacrificed. Their testes were dissected, fixed in 2% glutaraldehyde, post fixed in 2% osmium tetroxide. Finally, embedded in pure resin, prepared for ultrastructural study. For determination GSH, GPX, SOD, CAT, MDA and testosterone level, blood was obtained from heart. Female mice were superovulated and killed 24 hou inseminated with sperms for assessing fertilization rate. Data were analyzed by ANOVA and post hoc tests. Results: Determination of oxidative markers showed that the concentration of Catalase, GPX, MDA, SOD,GSH in experimental group, in comparison to other groups is reduced, while MDA level increased (p<0.001). Also serum testosterone level was decreased. Transmission electron microscopy was revealed in experimental group, in comparison to control group, cellular organels were decreased. Furthermore, detachment of basementmembrane from cytoplasmic membrane and a large number of vacuolar spaces in experimental was observed. Finally we found, BSOadministration caused a decrease in sperm fertilization capability. Conclusions: It is concluded that exposure to oxidative stress induced by buthionine sulfoximine (BSO) could affect ultrastructure of testis and sperm fertility. |
| نویسنده | نفر چندم مقاله |
|---|---|
| جعفر سلیمانی راد | دوم |
| لیلا روشنگر | سوم |
| علیرضا علی همتی | چهارم |
| محمد نوری | پنجم |
| فخرالسادات سجادیان | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Jokull Journal.pdf | 1398/10/24 | 1438951 | دانلود |
