چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Previous studies introduced ORZ for pharmacological purposes. As ORZ reduces NKs originated IFN-γ potentially. Therefore this study was designed to evaluate VEGF and MMP-9 expression in HEP-G2 cell line after culture with the soup of ORZ treated PBMCs. Materials and methods: In current in-vitro study, PBMCs from seven healthy male subjects (aged 20 to 30 years) were isolated by ficoll density gradient. The soup of ORZ treated PBMCs were cultured with HEP-G2 cell line and the VEGF and MMP-9 expression in the cancerous cell line were measured by real time PCR. Results: VEGF and MMP-9 expression increased after 48 hours, but only the increment of MMP-9 expression was statistically significant (P<0.05). The expression of these genes did not alter significantly after 24h and 72h incubation of the cancer cells with the soup of ORZ treated PBMCs soup (P>0.05). Conclusion: By considering the increase of MMP-9 and VEGF expression in the cancerous cell line cultured with ORZ-stimulated PBMCs soup, it seems long term administration of ORZ, as a drug, may change the response of immune system toward carcinogenesis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| میثم براتی | اول |
| مهدی یوسفی | دوم |
| حامد محمدی | سوم |
| مهرانگیز ابراهیمی ممقانی | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| MOJI-05-00159.pdf | 1397/08/15 | 529519 | دانلود |
