چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Staphylococcus aureus infections, particularly infections caused by methicillin-resistant S. aureus (MRSA) strains, are emerging as a major public health problem. The aim of the present study was to determine the prevalence of methicillin-resistant staphylococcus aureus (MRSA) by phenotypic and genotypic methods in clinical specimens and detection of the Panton – Valentine leukocidin (PVL) gene in the MRSA strains. In an 11-month study, 710 clinical specimens were collected from patients attending to several teaching hospitals of Urmia city, Northwest Iran. The isolates were examined by conventional culture method for detecting S. aureus strains and further confi rmation with standard biochemical tests, including catalase, coagulase and DNase. MRSA isolates phenotypically were screened by disk diffusion method. Then DNA was extracted from our MRSA isolates and mecA gene amplifi ed by PCR. Finally, pvl genes were identifi ed among MRSA isolates which were positive for mecA gene. Among test isolates, 114 isolates (16%) were confi rmed as S. aureus, from which 48 (42.1%) were recorded as MRSA. pvl gene was detected in 13 (27%) MRSA isolates. Our study showed that the prevalence of PVL-positive MRSA isolates, justify further detailed inspection to prevent possible future endemics in the studied hospitals and likewise other hospitals in the region |
| نویسنده | نفر چندم مقاله |
|---|---|
| جاوید صادقی | سوم |
| آیلین اسمعیل خانی | چهارم |
| محمدرضا نهائی | دوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Frequency of pvl.pdf | 1397/08/07 | 121209 | دانلود |
