چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| tOxidative stress possesses a key role in the onset and development of cardiovascular diseases (CVDs), thusit can be an efficient target to tackle such ailment. Marrubiin, a bioactive diterpene, is a potent antioxi-dant against oxidative stress. Herein, we aimed to formulate marrubiin loaded solid lipid nanoparticles(SLNs) to improve its pharmacokinetics and bioavailability and also to investigate free drug and for-mulation’s protective impact against intracellular reactive oxygen species (ROS) generation in HUVECs.Marrubiin-SLNs were formulated using hot homogenization/solidification method and then were sub-jected to physicochemical characterizations, i.e. size, zeta potential, morphology, polydispersity index(PDI), encapsulation efficiency (% EE), drug loading/content and physical stability assessments. MTT assaywas performed to study the cytotoxicity of the intact and SLN incorporated marrubiin on HUVECs. Further,the antioxidant property of marrubiin and formulations was evaluated using DPPH radical scavengingassay and their protective effect against TNF- induced oxidative stress was assessed by the meansof intracellular ROS assessment, and also apoptosis/necrosis, cell cycle, and DNA fragmentation assays.Electron microscopy analysis showed spherical monodispersed SLNs with the size less than 100 nm, par-ticle/zeta size analyses also approved the size of particles with a zeta potential of −1.28 ± 0.17 mV. Resultsalso showed high EE (98%), drug loading (31.74 mg/g) with 3.15% drug content. In vitro release studiesrevealed about 90% of marrubiin cumulative release during 24 h. The stability of marrubiin-SLNs in termsof size, zeta potential, polydispersity index, EE and drug leakage was approved. Marrubiin antioxidantstability after formulation was approved by DPPH analysis. MTT cell survival assay showed no significantcytotoxicity after 24 h and 48 h. Intracellular ROS detection assay revealed that marrubiin and marrubiin-SLNs, play protective effect against TNF- induced oxidative stress in HUVECs which was further approvedby apoptosis assessment. Conclusively, based on our findings, marrubiin nanoparticles are proposed as apreventive/therapeutic remedy against disorders elicited by increased levels of intracellular ROS in CV |
| نویسنده | نفر چندم مقاله |
|---|---|
| علیرضا گرجانی | هفتم |
| آیلار نخلبند | اول |
| مرتضی اسکندانی | دوم |
| نازلی سعیدی کوزه کنانی | سوم |
| صمد غفاری باویل | چهارم |
| یداله امیدی | پنجم |
| ژاله برار | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Marrubiin-loaded solid lipid nanoparticles impact on TNF treatedumbilical vein endothelial cells A study for cardioprotective effect.pdf | 1397/08/06 | 2115244 | دانلود |
