چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | حسین تقوی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز سلولهای بنیادی |
| کد مقاله | 64298 |
| عنوان فارسی مقاله | تعیین میزان بیان ژن های HMGB1 و SDF1 در داربست آسلولار بارگذاری شده با سلول های بنیادی مشتق از چربی و ماده مومیایی |
| عنوان لاتین مقاله | Determination rate of HMGB1 and SDF1 genes expression in the acellular scaffold Loaded with adipose derived stem cell and mummy substance |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | سیزدهمین کنگره علوم تشریحی |
| نوع کنگره / همایش | ملی |
| کشور محل برگزاری کنگره/ همایش | |
| شهر محل برگزاری کنگره/ همایش | همدان |
| سال انتشار/ ارائه شمسی | 1397 |
| سال انتشار/ارائه میلادی | 2018 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1397/07/25 الی 1397/07/27 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://13ancong.umsha.ac.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | taghavih@tbzmed.ac.ir |
| نویسنده | نفر چندم مقاله |
|---|---|
| حسین تقوی | اول |
| لیلا روشنگر | دوم |
| جعفر سلیمانی راد | سوم |
| احمد مهدی پور | پنجم |
| کبری ولائی | چهارم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | tissue engineering, Mummy substance, acellular scaffold, Adipose derived stem cell, HMGB1, SDF1 |
| خلاصه مقاله | Background and objective: Acellular scaffold extracted from extracellular matrix have been used for constructive and regenerative medicine.HMGB1 (high mobility group box1) has been reported to act as a danger signal in several disease states. It was shown that HMGB1 present in many biologic scaffolds.SDF1 (stromal cell derived factor_1) are important regulatory elements, which not only have protective effects against autoimmune diabetes, but also can enhance wound healing through recruiting bone marrow-derived mesenchymal stem cells and promoting revascularization. It was originally isolated from marine bone marrow stromal cells, and it is highly expressed on stromal cells of several tissues, including pancreas, spleen, ovary, and small intestine. The aim of this study wasestimating the rate of HMGB1 and SDF1 genes expression in the acellular scaffold Loaded with adipose derived stem cell and mummy substance. Materials and Methods: Decellularization of sheep jejunums was carried out using chemical reagents and various physical conditions. In orderto determine its morphology and the degree of decellularization, H&E stainingwas applied. Then, the scaffolds were treated with mummy substance and for characterized using FTIR, FE-SEM and UTM. ADCs were seeded on the scaffold and the morphology and adhesion of the cells to the scaffolds were assessed through FE-SEM at 24 and 48 h incubation.mRNA expression level was analyzed byRT_PCR.finally, the efficiency of mummy for inducing the proliferation of ADCson the scaffolds was evaluated usingMTT assay. Findings: Findings revealed satisfactory decellularization of jejunums which could be due to its thin thickness. H&E sections clearly showed that decellularization had removed all cell nuclei, but some cell nuclei appeared to be present in demineralized sections. FE-SEM showed that the mummy loaded and unloaded decellularized matrix possesses similar matrix morphology with a randomly oriented fibrillar structure and interconnecting pores. In RT_PCR significant increase was observedin HMGB1 and SDF1 expression level (p<0.001).No toxicity was observed in all treatments, and viability, expansion, and cell proliferation were supported in both scaffolds. Importantly, the proliferation capacity of ASCs on Mummy-loaded decellularized scaffold significantly increased in comparison with the unloaded scaffold after24 and 48 h incubation (P<0.001). Conclusion: The results of this study suggested that HMGB1 and SDF1 factors was expression in the acellular scaffold and expression level was elevated when scaffolds loaded with stem cell and mummy substance. Due to the importance of this genes in the Stimulating the immunologic factors and vascolurization, it seems that measurement this factors in acellular scaffolds is important. affiliation: stem cell research center, tabriz university of medical science |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| article A-10-253-1 (1).docx | 1397/08/01 | 17987 | دانلود |
| IMG_20181026_154634.jpg | 1397/08/05 | 1075764 | دانلود |
