چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Breast cancer remains a global challenge, and further chemopreventive therapies are still immediately required. Emerging evidence has revealed the potent anti-cancer effects of biguanides, Metformin (MET) and Phenformin (PHE). Thus, To explore an efficient chemopreventive strategy for breast cancer, the antiproliferative effects of the combination of MET and PHE against breast cancer cells were assessed in this work. Methods: Cytotoxic effects of the drugs individually and in combination against T47D and MDA-MB-231 breast cancer cells were assessed using MTT assay and the median-effect method was used to analyze the precise nature of the interaction between MET and PHE. Besides, the expression levels of hTERT after 48 h drug exposure was determined using qRT-PCR. Results: Based on the cytotoxicity assay, both MET and PHE further inhibited the growth of MDA-MB-231 cells compared with T47D cells. It was found that MET+PHE drastically reduced the IC50 values of MET and PHE in both cells than the single treatments in synergistic manner. Importantly, MET and PHE in combination form showed higher antiproliferative effect with smaller IC50 values against MDA-MB-231 cells than T47D cells. Real time PCR results revealed that hTERT expression was significantly reduced in both breast cancer cell lines treated with MET+PHE compared with the single treatments. In comparison between two types of breast cancer cells, it was detected that MET+PHE could further decline hTERT expression in MDA-MB-231cells than T47D cells (p<0.001). Conclusion: It is speculated that the combination of MET and PHE may be a promising and convenient approach to improve the efficiency of breast cancer treatment. |
| نویسنده | نفر چندم مقاله |
|---|---|
| داود جعفری قراباغلو | اول |
| یونس پیله ور سلطان احمدی | دوم |
| مهدی داداش پور | سوم |
| علی مطاع | چهارم |
| نصرت اله ضرغامی | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 99999977777 77777 3.pdf | 1397/07/10 | 654430 | دانلود |
