چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| یلدا جعفری متنق | اول |
| آزاده منتصری | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Key words: Osteoarthritis, chondrocyte, scaffold, Wharton’s jelly, stem cells |
| خلاصه مقاله | Abstract Background and aim: Osteoarthritis is the most common type of arthritis affected millions of people known as a second debilitating disease. Cartilage tissue engineering composed of different elements as cells, scaffold and chemical factors. Recently Wharton's jelly mesenchymal stem cells (WJSCs) has been introduced as a suitable source for repair of cartilage lesions. These cells have similarities to the cartilage extracellular matrix. Among different scaffolds, chitosan-gelatin scaffolds seem to be a good candidate because of their similarities to cartilage ECM and anti-bacterial properties. So, the aim of the present study is to investigate whether WJSCs can differentiate into chondrocyte on chitosan-gelatin scaffolds. Methods: Chitosan-gelatin scaffolds fabricated by Freeze-drying technique. WJSCs at fourth passage were delivered on to chitosan-gelatin scaffolds and then treated with chondrocyte differentiating medium for 10 days. Then after samples were prepared for evaluating gene expression profile using real time RT-PCR. Viability of WJSCs on this scaffolds also was evaluated using MTT technique. Attachment of cells to the scaffolds was discerned by SEM. Results: MTT assay technique revealed the viability of WJSCs on chitosan-gelatin scaffolds. High porosity of scaffolds was detected using scanning electron microscopy. Real time RT-PCR showed that the gene expression profile of collagen type II and Sox-9 was significantly higher compared to the monolayer culture. Conclusion: chitosan-gelatin scaffolds can be introduced as a good candidate for supporting the differentiation of WJSCs into chondrocytes. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 2.JPG | 1397/06/28 | 775467 | دانلود |
| 2 (2).JPG | 1397/06/28 | 1676558 | دانلود |
