Identification of nontuberculous mycobacteria isolated from hospital water by sequence analysis of the hsp65 and 16S rRNA genes
Identification of nontuberculous mycobacteria isolated from hospital water by sequence analysis of the hsp65 and 16S rRNA genes
نویسندگان: حسین صمدی کفیل , سید رضا مودب
کلمات کلیدی: Key words | 16S rRNA, hospital water, hsp65, nontuberculous mycobacteria
نشریه: 22162 , 15 , 5 , 2017
| نویسنده ثبت کننده مقاله |
سید رضا مودب |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
دانشکده پیراپزشکی |
| کد مقاله |
63616 |
| عنوان فارسی مقاله |
Identification of nontuberculous mycobacteria isolated from hospital water by sequence analysis of the hsp65 and 16S rRNA genes |
| عنوان لاتین مقاله |
Identification of nontuberculous mycobacteria isolated from hospital water by sequence analysis of the hsp65 and 16S rRNA genes |
| ناشر |
4 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
بلی |
| عنوان نشریه (خارج از لیست فوق) |
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| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
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| ABSTRACT
Nontuberculous mycobacteria (NTM) have emerged as an important cause of opportunistic
nosocomial infections. NTM has frequently been isolated from hospital water distribution systems.
The aim of this study was to survey the risk of NTM infections and determine the prevalence of NTM
species in the hospital water distribution systems in Tabriz, Iran. One hundred and twenty samples of
water from different sources of Tabriz hospitals were collected. The samples were filtered through
0.45-μm pore size membranes and decontaminated with 0.01% cetylpyridinium chloride. The
sediment was inoculated onto Lowenstein–Jensen medium and incubated for 8 weeks. For
identification to the species level, partial sequence analysis of the hsp65 and 16S rRNA genes were
used. NTM were detected in 76 (63.3%) of 120 samples. Potentially pathogenic mycobacteria and
saprophytic mycobacteria were isolated. Mycobacterium gordonae was the only single species that
was present in all types of water. The prevalence of NTM in Tabriz hospitals’ water compared with
many investigations on hospital waters was high. This indicates that the immunocompromised
patients and transplant recipients are at risk of contamination which necessitates considering
decontamination of water sources to prevent such potential hazards. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| Identification of nontuberculous mycobacteria isolated.pdf | 1397/05/17 | 342582 | دانلود |