چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| It is still unclear whether toll-like receptor signaling and serum levels of inflammatory markers in metabolically unhealthy abdominally obese (MUAO) are due to their obesity and/or their metabolic state. We hypothesized that abdominal obesity is an important mediator of the association of metabolic state with TLR signaling and serum inflammatory markers. Therefore, in this case control study, we compared the expression level of toll-like receptor4 (TLR4) and Toll/IL-1 receptor domain containing adaptor protein-inducing interferon-beta (TRIF)and serum concentrations of interferon β (IFNβ) and lipoprotein binding protein (LBP) in metabolically healthy abdominally obese (MHAO) and MUAO individuals. Basal blood samples from 65 abdominally obese subjects with waist circumference (WC)≥95cm were collected to determine serum metabolic parameters, INFβ, and LBP. Those with 3 or more metabolic alterations were defined as MUAO (n=34) and those having 2 or less were classified as MHAO (n=31). Furthermore, mRNA was isolated from peripheral blood mononuclear cells (PBMCs). TLR4 and TRIF gene expression assay was performed using quantitative real time-PCR (qPCR). There were significant differences in serum fasting blood sugar (p=0.017), triglyceride (p<0.001), cholesterol (p=0.002), and LDL-C (p=0.034) between MUAO and MHAO groups, whereas no significant difference was observed in the expression ratio of TLR4 and TRIF mRNA and serum levels of INFβ and LBP. However, a significant correlation was noticed between mRNA expression level of TLR4 and TRIF (r=0.50;p<0.001) and serum INFβ and LBP (r=0.70,p<0.001). It is concluded that the expression level of TLR4 and TRIF as well as serum INFβ and LBP are more related to abdominal obesity than to metabolic health. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهسا نقی زاده | اول |
| بهزاد برادران | دوم |
| مریم ثقفی اصل | سوم |
| پریچهر امیری | چهارم |
| داریوش شانه بندی | پنجم |
| ناهید کرم زاد | ششم |
| لیلا محامد خسروشاهی | هفتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Nutrition Research-main.pdf | 1397/05/16 | 1043041 | دانلود |
