چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Group B Streptococcus (GBS), as a risk factor for newborns, is one of the most important causes of meningitis and septicemia in newborns, intrauterine infections in women, and colonization of GBS in vaginal region in late gestation. The aim of this study was to determine GBS prevalence and to evaluate its infection among pregnant women through culture and Polymerase Chain Reaction (PCR) methods and, then, to compare these methods. To this end, vaginal and urine swabs were separately collected from 246 women at the women's hospital of Sari, Iran. Afterwards, samples were enriched in selective culture media Todd-Hewitt broth for 24 h at 370C, recognized using blood agar media, and, finally, were amplified STREP gene by PCR technique. The findings of this study indicated that the frequency of GBS in samples collected from urine and vaginal cultures and PCR method were positive 6.1% and 9.7%, respectively. In addition, no significant relationship was found among the positive results of culture, maternal age, gestational age, a history of abortion, and infection. With regards to the cultivation method as a standard method, the sensitivity of PCR test was 100% and the specificity was 96%. Moreover, it was found that the colonization rate of women GBS is very significant in Sari. Therefore, PCR method is recommended as a reliable and rapid method for detection of GBS. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مسعود مقدس زاده | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 2018,Current Sciences.pdf | 1397/02/19 | 1145620 | دانلود |
