چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Prostate cancer is known as the most common malignancy in men. Chitosan has generated great interest as a useful biopolymer for the encapsulation of small interfering RNA (siRNA). Due to cationic nature, chitosan is able to efficiently encapsulate siRNA molecules and form nanoparticles. Furthermore, the biocompatible and biodegradable attributes of chitosan have paved the way for its potential application in the in vivo delivery of therapeutic siRNAs. In this study, we aimed to design chitosan/CMD nanoparticles for the efficient encapsulation of the anti-cancer drugs SN38 and Snail-specific siRNA. Methods: Physicochemical characteristics, growth inhibitory properties, and anti-migratory capacities of the dual delivery of SN38-Snail siRNA CMD-chitosan nanoparticles were investigated in prostate cancer cells. Results: Our findings provided evidence for the suggestion that, ChNP-CMD-SN38-siRNA treated cells, the mRNA level of snail decreased from 1.00 to 0.30 (0.14) and 0.09 (0.04) after 24 h and 48 h, respectively. Additionally, the fold induction of E-cadherin and Claudin-1 increased from 1.00 to now 3.12 (0.62), 3.02 (0.28) after 24 h and 5.6 (0.91), 4.42 (0.51) after 48 h, respectively. Also, co-delivery of SN38 and Snailspecific siRNA by an appropriate nanocerrier (chitosan nanoparticles) could reduce the viability, proliferation, and migration of PC-3 cells. Conclusions: In conclusion, ChNPs encapsulating SN38 and Snail-specific siRNA may represent huge potential as an effective anti-cancer drug delivery system for the treatment of prostate cancer. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهدی یوسفی | یازدهم |
| لیلی عاقبتی | دوم |
| صنم صدرالدینی | چهارم |
| مجید احمدی | پنجم |
| صنم دولتی | ششم |
| پروین اکبرزاده لاله | هشتم |
| فرهاد جدیدی نیارق | نهم |
| وحید یونسی | دهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 1 (1).pdf | 1397/01/17 | 1783580 | دانلود |
