چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهدی مهدی پور | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Abstract: Proper oocyte nuclear and cytoplasmic maturation is an essential factor for developmental competence of the oocyte and subsequent development of a healthy embryo. Micro-RNAs (miRNA) are known for regulating gene expressions at both transcriptional and post-transcriptional levels and are involved in various biological processes such as control of cell cycle and apoptosis. The knowledge on importance of miRNAs in the oocyte is limited. In order to understand the functions of the miRNAs expressed during oocyte maturation, bovine oocytes at the germinal vesicle (GV) and metaphase II (MII) stages were collected and analyzed using next generation sequencing. In the total, 167 and 242 known miRNA species with more than 20 reads were identified for GV and MII oocytes respectively. The ratios of reads in MII to GV oocytes were calculated and the most differentially expressed miRNAs were miR-222, miR-199a, miR-221, miR-155, miR-214, Let-7b, miR-27a, miR-196a and miR-21. In-silico analysis revealed that target genes are involved in various cellular metabolic processes including protein modification like ubiquitination and phosphorylation as well as roles in the cell cycle and cell division. These results indicate that miRNAs play a significant role for preparation of the oocytes for the successful fertilization and subsequent embryonic development. Identification and quantification of miRNAs could be used to assess the developmental competence of an oocyte. The better understanding of mechanisms involved in miRNA and messenger RNA interaction in the oocyte may help us develop approaches for specific types of subfertility and contribute in the improvement of assisted reproductive technologies. |
| کلمات کلیدی | microRNA; next generation sequencing; oocyte quality; developmental competence |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Mahdipour et al..docx | 1396/12/24 | 15566 | دانلود |
| certificate.jpg | 1396/12/24 | 90009 | دانلود |
